DEAE Sephacel Column: Protein Purification via Ion Exchange

How do i decide on the pH of the column buffer if protein pI is unknown? Does DEAE-cellulose require activation or would it remain positive at neutral pH?

cellulose Columns)

deae cellulose column chromatography DEAE cellulose DE-52 · CAS No : 9013-34-7 · Level: column chromatography · Functional group: diethylaminoethyl · Normal pH range: · Small ion charge: 08

deae column chromatography The urine was filtered and the protein was concentrated by ultrafiltration After dialysis, a suitable aliquot was adsorbed on a DEAE cellulose column and